Study on Cryopreservation of Porphyra yezoensis Conchocelis     

ZHOU Wenjun LI Yun DAI Jixun 《中国海洋大学学报(英文版)》2007,6(3):299-302

Cryopreservation of Porphyra yezoensis conchocelis was conducted with cryoprotectants and a proposed pretreatment procedure and thawing methods explored. Six cryoprotectants combined by DMSO with ethylene glycol （EG）, propylene glycol （PEG）, sorbitol and sucrose were developed. The effect of prefreezing at -40℃ or -20℃ for different time durations was compared and the thawing methods were screened. It was shown that the cryoprotectant including 10% DMSO with 0.5 molL^-1 sorbitol exhibited the optimal effect. The ideal pretreatment was that conchocelis segments were stayed for 20min at -40℃ before stored in liquid nitrogen, and 40℃ water bath was proper for quick thawing. The highest recovery rate of cryopreserved P. yezoensis conchocelis reached 89.41%.  相似文献   

坛紫菜幼苗淋藏换网养殖试验     

叶金聪 《台湾海峡》2009,28(2):293-298

利用坛紫菜强耐干特性,通过适当的淋水使幼苗在常温下较长时间保藏,从而达到换网养殖的目的.试验结果得出,从自然海区取回苗网,在室内常态下每天淋水2次,每次1h,经52d左右后下海挂养,尚有3～4个月的养成期,产量可达到、甚至超过传统生产期的总产量.这一方法的推广应用有望大幅度提高我国坛紫菜生产总产量.  相似文献   

Sequence analysis of the ITS region and 5.8S rDNA of <Emphasis Type="Italic">Porphyra haitanensis</Emphasis>     

李艳燕  沈颂东  贺丽虹  许璞  王广策 《中国海洋湖沼学报》2009,27(3):493-501

The sequences of the ITS(internal transcribed spacer) and 5.8S rDNA of three cultivated strains of Porphyra haitanensis thalli(NB,PT and ST) were amplified,sequenced and analyzed.In addition,the phylogenic relationships of the sequences identified in this study with those of other Porphyra retrieved from GenBank were evaluated.The results are as follows:the sequences of the ITS and 5.8S rDNA were essentially identical among the three strains.The sequences of ITS1 were 331 bp to 334 bp,while those of the 5.8...  相似文献   

坛紫菜胞质型抗坏血酸过氧化物酶基因的克隆及特征分析     

张晓龙  杨锐  仪茜茜  孙雪  吴晓微  王亚军 《海洋学报(英文版)》2010,29(5):165-174

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Cloning and Analysis of Calmodulin Gene from Porphyra yezoensis Ueda (Bangiales, Rhodophyta)     

WANG Mengqiang）  ））  MAO Yunxiang）  *  ZHUANG Yunyun）  ）  KONG Fanna）    SUI Zhenghong） ） College of Marine Life Sciences  Ocean University of China  Qingdao  P. R. China ） Institute of Oceanology  Chinese Academy of Sciences  Qingdao  P. R. China ） Graduate University of Chinese Academy of Sciences  Beijing  P. R. China ） Department of Marine Sciences  University of Connecticut  Groton CT  USA 《中国海洋大学学报(英文版)》2009,8(3)

In order to understand the mechanisms of signal transduction and anti-desiccation mechanisms of Porphyra yezoensiss,cDNA and its genomic sequence of Calmodulin gene (CaM) was cloned by the technique of polymerase chain reaction (PCR) based on the analysis of P. yezoensis ESTs from dbEST database. The result shows that the full-length cDNA of CaM consists of 603 bps including an ORF encoding for 151 amino acids and a terminate codon UGA, while the length of genomic sequence is 1231 bps including 2 exous and 1 intron. The average GC content of the coding region is 58.77%, while the GC content of the third position of this gene is as high as 82.23%. Four Ca2+ binding sites (EF-hand) are found in this gene. The predicted molecular mass of the deduced peptide is 16688.72 Da and the pI is 4.222. By aligning with known CaM genes, the similarity of CaM gene sequence with homologous genes in Chlamydomonas incerta and Chlamydomonas reinhardtii is 72.7% and 72.2% respectively, and the similarity of the deduced amino acid sequence of CaM gene with homologous genes in C. incerta and C. reinhardtii are both 71.5%. This is the first report on CaM from a species of Rhodophyta.  相似文献   

五种紫菜同工酶标记的遗传分析     

沈颂东  李艳燕  许璞  张美如  袁昭兰  王建伟  朱建一 《海洋通报(英文版)》2010,12(1):65-78

用垂直凝胶电泳来研究采自中国的（条斑紫菜×坛紫菜）杂交种、坛紫菜、条斑紫菜、少精紫菜、半叶紫菜的同工酶多态性。然后分析最小可能位点数和观察同位基因数、遗传差异性和采用平均连接聚类法。选取23种酶带中的六个酶（MDH，ME，LDH，GDH，IDH和G-6-PDH）进行分析。最小可能等位基因位点数显示五种紫菜在ME等位基因位点都只有一个。LDH和GDH等位基因位点半叶紫菜和少精紫菜和另外的三种紫菜不同，而在分析MDH时半叶紫菜比较独特。在IDH位点分析时少精紫菜和坛紫菜和其他三种不同，而条斑紫菜和坛紫菜和其他三种不同在G-6-PDH位点不一致。总的来看，最小可能等位基因位点数分析半叶紫菜是最分离的。遗传多样性结果分析表明当遗传相似度为0．7550时，五种紫菜中的遗传变异研究受到限制。条斑紫菜和坛紫菜的杂交种非常接近少精紫菜。当遗传相似度达到0．8937时，出乎意料的是条斑紫菜和坛紫菜遗传同一。性很低。条斑紫菜4个株系的平均遗传相似度仅为0．7428，差异比较大。在所有紫菜中半叶紫菜是最分化的分支，它与最小可能等位基因位点数分析一致。  相似文献   

CO₂升高和阳光紫外辐射对坛紫菜生长和光合特性的耦合效应   总被引：1，自引：0，他引：1  

徐军田  高坤山 《海洋学报》2013,35(5):184-190

大气CO₂持续升高,导致溶入海水中的CO₂增多,海水表层的H+浓度增加,从而引起海洋酸化。为了探讨近岸定生大型海藻对这种环境变化的响应,本文选择经济海藻坛紫菜为实验材料,研究海洋酸化与紫外辐射对藻体生长以及光合特性的影响。实验分两个CO₂处理,分别为正常空气水平(390 ppmv)和高CO₂水平(800 ppmv); 三种辐射处理,分别为全波长辐射(PAB)、滤除紫外线B(PA)和仅接受可见光处理(PAR)。研究结果表明,CO₂培养下的坛紫菜,在仅有可见光(P)或者同时有紫外线A(PA)存在的情况下,显著促进藻体的生长;但在全波长辐射处理下(PAB),这种作用不明显。高CO₂降低了藻体在P和PA处理下的光合作用速率,但对PAB处理作用不显著。高CO₂处理下的藻体,UV-B显著降低了全波长辐射下藻体紫外吸收物质的含量,但在正常CO₂水平下,紫外辐射的作用不显著。这表明高CO₂导致的生长优势被紫外辐射的负面效应所抵消,在全球变化的过程中,紫外辐射的进一步加强在海洋酸化的背景下甚至有可能降低坛紫菜的产量。  相似文献   

Pyropia yezoensis can utilize CO2 in the air during moderate dehydration     

周伟  何林文  杨芳  林阿朋  张宝玉  牛建峰  王广策 《中国海洋湖沼学报》2014,32(2):358-364

Pyropia yezoensis,an intertidal seaweed,experiences regular dehydration and rehydration with the tides.In this study,the responses of P.yezoensis to dehydration and rehydration under high and low CO 2 concentrations((600–700)×10-6 and(40–80)×10-6,named Group I and Group II respectively)were investigated.The thalli of Group I had a signifi cantly higher effective photosystem II quantum yield than the thalli of Group II at 71% absolute water content(AWC).There was little difference between thalli morphology,total Rubisco activity and total protein content at 100% and 71% AWC,which might be the basis for the normal performance of photosynthesis during moderate dehydration.A higher effective photosystem I quantum yield was observed in the thalli subjected to a low CO 2 concentration during moderate dehydration,which might be caused by the enhancement of cyclic electron fl ow.These results suggested that P.yezoensis can directly utilize CO 2 in ambient air during moderate dehydration.  相似文献   

海州湾条斑紫菜养殖对浮游藻类群落结构及遗传多样性的影响     

王洪斌  李信书  宋秀梅  吴言  李士虎  阎斌伦 《海洋科学》2014,38(12):103-109

为了研究条斑紫菜(Porphyra yezoensis)养殖对江苏海州湾浮游藻类群落结构及遗传多样性的影响,于江苏省海州湾条斑紫菜养殖及非养殖海区各设置4个采样位点,采集表层海水,提取海水中浮游藻类的总DNA,PCR扩增浮游藻类核酮糖1,5-二磷酸羧化/氧化酶大亚基(rbc L)基因片段,构建了养殖及非养殖海区rbc L片段质粒文库。在文库中随机选择50个阳性克隆并测序。经比对分析,在条斑紫菜非养殖区发现8种海洋微藻,隐鞭藻占总克隆数22%,海链藻和骨条藻分别占6%和2%;养殖海区发现10种微藻,其中异丝藻占总克隆数22%,优势度明显,仅3种微藻为二海区共有(骨条藻、隐鞭藻及小球藻),表明紫菜养殖及非养殖区浮游藻类群落组成差异显著。条斑紫菜非养殖区及养殖区的浮游藻类香农指数均值分别为3.273和3.654,均匀度指数均值分别为1.090和1.040,显示养殖区浮游藻类遗传多样性较丰富,而群落的成熟度与稳定性非养殖区高。研究证实,表层海水浮游藻类群落结构及组成是动态的,条斑紫菜的养殖行为形成浮游藻类群落结构及遗传多样性较大差异性。  相似文献   

Exploring valid internal-control genes in Porphyra yezoensis （Bangiaceae） during stress response conditions     

王文磊  吴晓洁  王超  贾兆君  何林文  韦一凡  牛建峰  王广策 《中国海洋湖沼学报》2014,32(4):783-791

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